Dissertation
Evolution of gene expression in the cell-division cycle of woodland populations of budding yeast
Gene transcription events compose the primary decoding of an organism’s genetic program, forming the basis of phenotypic expression. Although changes in the regulation of gene expression have broad implications for the evolution of many basic biological phenomena, such as cell growth and proliferation, body plan formation, and environmental and genetic robustness, understanding which and how natural evolutionary processes affect gene expression remain largely unanswered, due to the nature of transcription as a complex quantitative trait. Moreover, as transcription is a dynamic process, with expression levels changing throughout an organism’s development, evolution likely influences a gene’s expression differentially as a function of time. Taking a comparative genomic approach, this dissertation aims to evaluate empirically transcriptome evolution in natural, woodland strains of the budding yeast Saccharomyces cerevisiae, during the mitotic cell-division cycle.
My Kim Lab Log
Refer to the lab resources page for a collection of lab protocols and various resources relating to budding yeast.
This site is also home to my ditigal research log. Feel free to check it occasionally.
Occassionally I also post paper summaries of some of the articles I have read.
KimLab Rotation project
My rotation project consisted of predicting yeast gene expression levels, using a so-called basis set of the most upstream transcription factors (i.e. those transcription factors that regulate every other TF or gene). In case you would like to pick up on it, or are generally interested, I have a bunch of code and information about the project. Here are two fairly outdated items: GEXP paper, presentation.
