Penn Program in Single Cell Biology

Workshops

Smart-Seq and Library Preparation Workshop

Rapidly amplify single cell RNA using SMART-seq2 technology and prepare libraries for RNA-seq. Learn about the mechanisms behind the techniques and the advantages of working with single cells.

Techniques include

  • Single cell isolation - patch pipette, Isoraft
  • RNA amplification - SMART-seq2
  • Library preparation – Illumina Nextera DNA Library Preparation Kit
  • RNA-seq data analysis using PIVOT

Dates: Tuesday, August 15 – Wednesday, August 16, 2017
Schedule: 9am - 5pm daily
Location: Room 24, John Morgan Building
Cost: $500
Open to: Penn faculty, postdocs and graduate students
Application deadline: Tuesday, August 1, 2017

Places are limited. To apply, complete the application form (available here) and email it, along with a two-page CV, to Jifen Li. Successful applicants will be notified on Monday, August 7.

Contact: Jifen Li, PhD, Associate Director of Programs, PPSCB
jifenli@mail.med.upenn.edu

Sponsors: ITMAT and Penn Program in Single Cell Biology



Previous Workshops

Workshop on Single Cell Transcriptomics for Cellular Phenotyping

Could single cell transcriptomics advance your research?

Learn high throughput single-cell RNA sequencing technologies from leaders in the field at the University of Pennsylvania’s Penn Program in Single Cell Biology.

Techniques include:

  • Single cell isolation - patch pipette, Isoraft, Fluidigm C1
  • RNA amplification - linear amplification (aRNA), Smart-seq
  • Image-based methods (TIVA, TISA)
  • RNA-seq library preparation (Illumina TruSeq Stranded and Nextera Kit)
  • RNA-Seq data analysis (KimLabIDV)

Dates: Monday, March 13 – Friday, March 17, 2017
Location: The University of Pennsylvania Perelman School of Medicine
Workshop fee: $300
Dormitary housing: $50/night with promotional code PPSCB2017 at www.ihousephilly.org
Course Instructors: Drs. James Eberwine, Junhyong Kim, Jifen Li and others.
Application deadline: Friday, February 3, 2017

Sponsors: NHGRI, ITMAT, Penn Program in Single Cell Biology, Illumina and Clontech Laboratories.


Single Cell Transcriptomics Workshop

The PENN Program in Single Cell Biology is offering a workshop on single cell transcriptome analysis using linear aRNA amplification and SMART-seq.

Techniques include

  • Single cell isolation - patch pipette, Isoraft
  • RNA amplification - linear amplification (aRNA), Smart-seq2
  • Preparation of single cell libraries for Illumina sequencing
  • RNA-Seq data analysis using KimLabIDV

Dates: Monday, January 23 – Friday, January 27, 2017
Schedule: 9am - 5pm daily
Location: Room 24, John Morgan Building
Cost: $500
Open to: Penn faculty, postdocs and graduate students
Application deadline: Friday, January 13, 2017

Sponsors: ITMAT, Penn Program in Single Cell Biology, Illumina and Clontech Laboratories.

Smart-Seq and Library Preparation Workshop

Learn to use the SMART-seq2 protocol to generate sequencing libraries from single cells.

Techniques include

  • Single cell isolation - patch pipette, Isoraft
  • RNA amplification - Smart-seq2
  • Library preparation – Illumina Nextera DNA Library Preparation Kit
  • Single-cell gene expression analysis using KimLabIDV

Dates: Tuesday, August 16 – Wednesday, August 17, 2016
Schedule: 9am - 5pm daily
Location: Room 24, John Morgan Building
Cost: $500
Open to: Penn faculty, postdocs and graduate students
Application deadline: Monday, August 1, 2016

From Single Cell to RNA-Seq

Could single cell expression profiling advance your research?

The PENN Program in Single Cell Biology is offering a workshop on linear amplification of RNA (aRNA) from single cells and sample preparation for RNA-Seq.

  • Learn on primary neurons
  • Bring your own samples (in consultation with Dr. Li)
  • Harvest single cells using a patch pipette
  • Produce aRNA from each cell using linear amplification
  • Prepare single cell libraries for Illumina sequencing
  • Learn how the techniques work and how single cell biology can augment your research
  • Generate preliminary data for your grant proposal

Dates: Monday, May 9 – Friday, May 13, 2016
Schedule: 9:00am – 5:30pm daily
Cost: $500
Open to: Penn faculty, postdocs and graduate students.
Application deadline: Friday, April 22, 2016

National Single Cell Transcriptomics Workshop

Learn advanced techniques for single cell isolation, RNA amplification and gene expression profiling by RNA-seq from leaders in the field at the University of Pennsylvania’s Penn Program in Single Cell Biology.

The data that participants collected during the workshop is available for public download here.

Techniques include:

  • Single cell isolation - patch pipette, Isoraft, Fluidigm C1
  • RNA amplification - linear amplification (aRNA), Smart-seq
  • Image-based methods
  • RNA-seq library preparation
  • Basic informatics

Participants may bring their own samples, on consultation with course instructors.

Dates: Mon, 4th May – Fri, 8th May 2015
Location: The University of Pennsylvania
Workshop fee: $300
Dormitary housing: $300
Course Instructors: Dr. James Eberwine, Dr. Junhyong Kim, Dr. Catherine Moorwood and others.
Application deadline: Fri, 6th Mar, 2015

Sponsors: NHGRI, ITMAT, Penn Program in Single Cell Biology, Illumina, Clontech Laboratories and Fluidigm.

Smart-Seq and Libraries Workshop

Rapidly amplify single cell RNA using Smart-seq technology and prepare libraries for RNA-seq. Learn about the mechanisms behind the techniques and the advantages of working with single cells. Participants may bring their own samples (on consultation with Dr. Moorwood). If you wish to do this, and have not isolated single cells before, the Single Cell Isolation Workshop (see above) is recommended!

Dates: Thu, 19th Mar - Fri, 20th Mar, 2015
Times: 9am - 5pm daily
Location: Room 24, John Morgan Building
Instructors: Dr. James Eberwine, Dr. Junhyong Kim, Dr. Catherine Moorwood
Open to: Penn faculty, trainees, senior research investigators and graduate students
Workshop fee: $400
Application deadline: Fri, 13th Feb 2015

Single Cell Isolation Workshop

Bring your own samples (on consulation with Dr. Moorwood) and learn how to isolate single cells using a patch pipette or Cell Microsystems Isoraft technology.

Date: Fri, 13th Mar, 2015
Time: A time slot will be assigned to each participant
Location: Room 37, John Morgan Building
Instructor: Dr. Catherine Moorwood
Open to: Penn faculty, trainees, senior research investigators and graduate students
Workshop fee: $50
Application deadline: Fri, 13th Feb 2015

Linear RNA Amplification (aRNA) Workshop

The PENN Program in Single Cell Biology is offering a workshop on linear amplification of RNA (aRNA) from single cells and sample preparation for RNA-Seq.

  • Bring your own samples (upon consultation with Dr. Moorwood)
  • Harvest single cells using a patch pipette or Cell Microsystems Isoraft technology
  • Produce aRNA from each cell using linear amplification
  • Prepare single cell libraries for Illumina sequencing
  • Learn how the techniques work and how single cell biology can augment your research
  • Generate preliminary data for your grant proposal

Dates: Monday, 1st Dec – Friday, 5th Dec 2014
Schedule: 9am – 5:30pm daily
Location: Room 24, John Morgan building
Cost: $500
Open to: Penn faculty, postdocs and graduate students
Application deadline: Friday, 31st Oct 2014