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Comparative yeast time-series gene expression

Maintained by Daniel Simola

License (pdf)

Citation

Heterochronic evolution reveals modular timing changes in budding yeast transcriptomes

Daniel F Simola, Chantal Francis, Paul D Sniegowski, Junhyong Kim
Genome Biology 2010, 11:R105 (22 October 2010)

Download the expression data

Normalized data are stored as tab-delimited text files. Raw data are stored in SAS (one data point per-line) format.

• Natural yeast line expression (normalized+imputed)
• Natural yeast line expression (RAW)
• Mutation accumulation line expression (normalized+imputed)
• Mutation accumulation line expression (RAW)
• Reference comparisons (RAW)

Map files for expression data

• Map for woodland lines
• Map for mutation accumulation lines
• Map for reference comparisons

GAL files

• Yeast Oligo v1.1 24 Pins (03-19-07).gal
• Yeast-gal_file7-2006.txt
• YeastOligoChip_08_29_2005.gal.txt

Platform information

Our microarrays are printed using the Operon AROS v1.1 for S. cerevisiae platform. For more information, see Operon's web page.

• yeast_V1.1_384.xls

Protocol

Include methods for cDNA synthesis from RNA, Dye coupling, slide preparation, hybridization, slide cleanup, and reagent list.

• KimLab_yeast_microarray_protocol.pdf